Ihsan R. Ibrahim

Abstract

The aim of this study was to determine the toxic effects of manganese in the thyroid gland and some of the biochemical and hematological parameters as well as assess the role that could be
played by each of selenium and iron to reduce the toxic effects of manganese in male rats for four weeks. Use in this study (35) an animal of male rats were divided randomly into five groups , the first group(C): it as control group was given saline physiological and given bush normal , the second group( G1): that given manganese chloride concentration of 150 mg / kg of body weight per day , the third group( G2): which given manganese chloride concentration of 150 mg / kg of body weight was also given sodium Selenate concentration of 0.5 mg / kg of diet per day , the fourth group( G3): which given manganese chloride concentration of 150 mg / kg body weight was also given ferrous sulfate concentration of 30 mg / kg of diet per day , the fifth group( G4): that given manganese chloride concentration of 150 mg / kg of body weight was also given sodium Selenate concentration of 0.5 mg / kg of diet and ferrous sulfate concentration of 30 mg / kg of diet daily . After the end of experiment were studied the following parameters : the hormonal parameters included (Thyroxin hormone T4 and Triiodothyronine hormone T3 , Thyroid stimulating hormone TSH) and liver enzymes included (AST, ALT, ALP) and hematological parameters included (red blood cells count ,hemoglobin concentration and packed cell volume ) in male rats . The results showed that given manganese chloride in rats has led to a significant decrease (P <0.05) in the level of the Thyroxin hormone T4 and Triiodothyronine hormone T3 with a significant increase in the level of thyroid stimulating hormone TSH in study.a significant increase (P <0.05) in the level of enzymes of ALT, AST and ALP in the term of study.a significant decrease (P <0.05) in the rate of red blood cells count and the hemoglobin concentration and packed cell volume for a period of four weeks . Animals that given of Sodium Selenate and ferrous sulfate separately or both, and simultaneously with manganese chloride in groups G4, G3, G2 has led to obtain a significant improvement in studied parameters compared with the group G1 which given manganese chloride and soon sometimes for control group, especially in the group G4 , which given the best results due to the synergistic action of elemental selenium and iron in reducing the toxic effects caused by manganese .