Pathological study of Stachybotrys chartarum that isolated from baths floor in Babylon province on some respiratory and digestive organs in white mice(Balb/c)
Keywords:
: Stachybotrys chartarum, white mice
Abstract
Stachybotrys chartarum is filamentous fungi that cause respiratory system infection in human and animals . This study was aimed to evaluate histological changes in some respiratory and digestive organs such as pharynx, trachea and lung induced by this fungus suspension . Detection of Stachybotrys chartarum fungus confirmed by cultured random samples that collected in clean containers from baths floor on potato dextrose agar in room temperature for 7-14 days. Then the suspension of positive samples of this fungus diluted by normal saline and counted the spores by using hemocytometer before used experimentally in lab animals (Balb mice) to study histological changes in some respiratory and digestive organs . Twelve mice used in this study divided into two groups . first group consist of six mice injected orally with 0.5 ml from one positive sample of fungus suspension contain 2×103 spore / mm3(for month : one dose daily) according to count of hemocytometer to evaluate some pathological changes in pharynx ,trachea and lung .Second group injected orally with 0.5 ml from phosphate buffer saline only. The results revealed that no changes in the pharynx of mice infected with0.5 ml contain 2×103 spore / mm3 from Stachybotrys chartarum fungus suspension. the lung of group mice treated with same concentration of fungus has interstitial pneumonia with thickening of inter alveolar space due to infiltration and chronic inflammatory cells especially mononuclear cell. While the trachea of group mice treated with same concentration of fungus suffering sub mucosal chronic inflammatory cells and infiltration with increase thickness of mucosal basal layer .Downloads
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References
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12- Nikulin, M.; Reijula, K.; Jarvis, B.B.; Veijalainen, P and Hintikka, E.-L. (1997). Effects of intranasal exposure to spores of Stachybotrys atra in mice. Fundam Appl Toxicol, 35:182-188.
2- Samson ,RA .; Houbraken ,J.; Thrane ,U.; Frisvad ,JC and Andersen B. (2010). Food and Indoor Fungi. CBS-KNAW- Fungal Biodiversity Centre, Utrecht, the Netherlands. pp. 1-398
3-Andersen ,B; Frisvad ,JC; Søndergaard ,I ; Rasmussen, IS and Larsen ,LS. (2011). Associations between fungal species and water damaged building materials. Applied and Environmental Microbiology.
4-Heller ,RM ; Heller ,TW and Sasson ,JM .(2003). "Mold: history of a confusion". Perspect. Biol. Med. 46 (4): 588–91.
5-Page, E. H and Trout, D. B. (2001). "The Role of Stachybotrys Mycotoxins in Building-Related Illness". AIHAJ - American Industrial Hygiene Association 62 (5): 644.
6-Gao ,P and Martin ,J. (2002). "Volatile metabolites produced by three strains of Stachybotrys chartarum cultivated on rice and gypsum board". Appl. Occup .Environ Hyg. 17 (6): 430–6.
7-Andersen B, Nielsen KF, Thrane U, Szaro, T, Taylor, JW and Jarvis, BB. (2003). Molecular and phenotypic descriptions of Stachybotrys chlorohalonata sp. nov. And two chemotypes of stachybotrys chartarum found in water-damaged buildings. Mycologia 95: 1227-1
8-Scott, A. Masten (2004). Stachybotrys chartarum . National Toxicology Program (NTP) .National Institute of Environmental Health Sciences (NIEHS).
9-Luna ,L.G.( 1968). Manual of histological staining methods of the Armed Forces Institute of Pathology, 3rd Ed. McGraw Hill. New York. 195 - 196.
10-Korpi, A; Kasanen, J.P; Raunio, P; Kosma, V.M ; Virtanen, T., and Pasanen, A.L. (2002). Effects of aerosols from nontoxic Stachybotrys chartarum on murine airways. Inhal .Toxicol, 14:521-540.
11-Leino, M.; Makela, M.; Reijula, K.; Haahtela, T.; Mussalo-Rauhamaa, H.; Tuomi, T.; Hintikka, E.-L and Alenius, H. (2003). Intranasal exposure to a damp building mould, Stachybotrys chartarum, induces lung inflammation in mice by satratoxin-independent mechanisms. Clin Exp . Allergy, 33:1603-1610.
12- Nikulin, M.; Reijula, K.; Jarvis, B.B.; Veijalainen, P and Hintikka, E.-L. (1997). Effects of intranasal exposure to spores of Stachybotrys atra in mice. Fundam Appl Toxicol, 35:182-188.
Published
2018-01-17
How to Cite
K . Al-Mansoori, A. (2018). Pathological study of Stachybotrys chartarum that isolated from baths floor in Babylon province on some respiratory and digestive organs in white mice(Balb/c). Al-Qadisiyah Journal of Pure Science, 22(2), 170-176. Retrieved from https://journalsc.qu.edu.iq/index.php/JOPS/article/view/579
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